Generator

Part:BBa_K195411

Designed by: Syue-Liang Lin   Group: iGEM09_NYMU-Taipei   (2009-10-21)

pTetR+RBS+PelB+F10scFv+C-IgAP+Term

E.coli is Gram-negative bacteria that have the inner (IM) and outer (OM) membranes ,and the periplasmic space contains the peptidoglycan layer.

The leader sequence signal peptide(PelB) was cleaved at the inner membrane, releasing the mature protein (downstream of PelB)into the periplasmic space . In the periplasmic space, the beta-domain(C-terminal) of the protein(IgAP) inserts into the outer membrane to form a β-barrel structure. After formation of the β-barrel pore, the N-passenger domain(F10scFv) was translocated to the cell surface through the pore.

So we can anchor the F10scFv(antibody) on E.coli 's surface,and catch the influenza A virus.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1107
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 134
    Illegal NgoMIV site found at 1118
    Illegal NgoMIV site found at 1743
    Illegal AgeI site found at 1518
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 999


[edit]
Categories
Parameters
None